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Developmental Biology

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JoVE Journal Developmental Biology
Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
 

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Article DOI: 10.3791/59823-v • 09:00 min • June 16th, 2019
June 16th, 2019 •
*1, *1, 1, 1
1Center for iPS cell research and application (CiRA)
* These authors contributed equally

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résumé
Automatic Translation

Here, we present a protocol to precisely form hemogenic endothelium from human pluripotent stem cells in a feeder- and xeno-free condition. This method will have broad applications in disease modeling and screening for therapeutic compounds.

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Hemogenic Endothelium Differentiation Human Pluripotent Stem Cells Feeder-free Xeno-free Defined Condition Blood Cell Production Consistent Yields Precise Yields Disease Monitoring Therapeutic Compounds Screening Hematology Research Immunology Research Genetic Intervention Gene Editing LM511-E8 Coating PSC Colonies Typing Visual Demonstrations Dissociation Buffer LM511-E8-coated Six-well Plate MTeSR1 Medium Hemogenic Induction PBS Wash Dissociation Solution PSC Maintenance Medium Microcentrifuge Tube Centrifugation
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