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Developmental Biology

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JoVE Journal Developmental Biology
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
 

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Article DOI: 10.3791/57795-v • 11:29 min • September 6th, 2018
September 6th, 2018 •
*1, *1, 1, 1, 1
1Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France
* These authors contributed equally

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résumé
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The production of specialized retinal cells from pluripotent stem cells is a turning point in the development of stem cell-based therapy for retinal diseases. The present paper describes a simple method for an efficient generation of retinal organoids and retinal pigmented epithelium for basic, translational, and clinical research.

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Xeno-free Feeder-free Culture Conditions Generation Human IPSC-derived Retinal Cell Models Differentiation Pluripotent Stem Cell Retinal Field Retinal Cell Types Human-induced Pluripotent Stem Cell IPS Cells Retinal Organoids Laboratory Engineer Amelie Slembrouck-Brec Celine Nanteau Retinal Pigmented Epithelial RPE Cell Production IPS Cell Differentiation Colonies Confluence Basal IPS Medium Basal IPS N-2 Medium Neuroepithelium Buds Pro B-27 Medium
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