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Neuroscience

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JoVE Journal Neuroscience
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
 

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Article DOI: 10.3791/53247-v • 09:27 min • September 14th, 2015
September 14th, 2015 •
*1,2,3,4,5, *1,2,3,4, 1,2,3,4, 1,2,3,4, 5, 1,2,3,4, 1,2,3,4
1CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, 2INSERM, UMR 967, 3Université Paris Diderot, Sorbonne Paris Cité, UMR 967, 4Université Paris Sud, UMR 967, 5CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department
* These authors contributed equally

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résumé
Automatic Translation

We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.

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Keywords: Neural Stem Cells Subventricular Zone Cell Sorting Fluorescence-activated Cell Sorting (FACS) Cell Cycle Dynamics Transit Amplifying Cells Neuroblasts FUCCI Live-imaging Aging Neurogenesis
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