JoVE Journal
Developmental Biology
Developmental Biology
Ce contenu est en libre accès.
Chapitres
résumé
Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.