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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
 

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Article DOI: 10.3791/53723-v • 11:44 min • January 24th, 2016
January 24th, 2016 •
*1,2,3, *1,3, 1, 1,2,3
1CRCHUM - Research center, Centre Hospitalier de l'Université de Montréal, Université de Montréal, 2Department of Biochemistry and Molecular Medicine, Université de Montréal, 3Faculty of Medicine, Université de Montréal
* These authors contributed equally

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résumé
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Here we describe a procedure allowing a detailed analysis of the phosphorylation-dependent activation of the IRF3 transcription factor. This is achieved through the combination of a high resolution SDS-PAGE and a native-PAGE coupled to immunoblots using multiple phosphospecific antibodies.

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High Resolution Method Monitor Phosphorylation-dependent Activation IRF3 Transcription Factor Innate Immune Response Interferon Beta Expression Pathogens Pathogen-associated Molecular Patterns A549 Cells Culture Infection Trypsinize Centrifuge Cell Pellet Single-cell Suspension
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