Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / One-step Negative Chromatographic Purification of …
JoVE Journal
Immunology and Infection

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Immunology and Infection
One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
 

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode

Article DOI: 10.3791/54043-v • 10:44 min • June 18th, 2016
June 18th, 2016 •
1, 1, 1, 1, 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

Chapitres

résumé
Automatic Translation

A high yield method for one-step negative purification of recombinant Helicobacter pylori neutrophil-activating protein (HP-NAP) overexpressed in Escherichia coli by using diethylaminoethyl resins in batch mode is described. HP-NAP purified by this method is beneficial for the development of vaccines, drugs, or diagnostics for H. pylori-associated diseases.

Tags

Keywords: Helicobacter Pylori Neutrophil-activating Protein HP-NAP Escherichia Coli Negative Chromatographic Purification Batch Mode DEAE Ion Exchange Resin Protein Purification Aggregation Prevention Bradford Assay
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter