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Journal / Biology / FRET Imaging i Tre-dimensionelle Hydrogeler
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Biology

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FRET Imaging i Tre-dimensionelle Hydrogeler
 
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FRET Imaging i Tre-dimensionelle Hydrogeler

Article DOI: 10.3791/54135-v • 09:47 min • August 1st, 2016
August 1st, 2016 •
*1, *1,3, 1,2
1Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 2Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 3Laerdal AS
* These authors contributed equally

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Förster resonans (FRET) imaging er et kraftfuldt værktøj til realtid cellebiologiske undersøgelser. Her en fremgangsmåde til FRET billeddannelse celler i fysiologiske tredimensionale (3D) hydrogel mikromiljøer anvendelse af traditionel epifluorescensmikroskopi præsenteres. En analyse for ratiometriske FRET sonder, der giver lineære nøgletal over aktiveringen sortiment er beskrevet.

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Molecular Biology Mikrofluid microtissue fluorescens Förster stillads polyethylenglycol hydrogel FRET intracellulær signalering chondrocytter
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