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Bioengineering

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JoVE Journal Bioengineering
Micropatterning and Assembly of 3D Microvessels
 

Micropatterning and Assembly of 3D Microvessels

Article DOI: 10.3791/54457-v • 13:05 min • September 9th, 2016
September 9th, 2016 •
*1, *1, 2, 1,2
1Department of Bioengineering, University of Washington, 2Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington
* These authors contributed equally

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résumé
Automatic Translation

This manuscript presents an injection molding method to engineer microvessels that recapitulate physiological properties of endothelium. The microfluidic-based process creates patent 3D vascular networks with tailorable conditions, such as flow, cellular composition, geometry, and biochemical gradients. The fabrication process and examples of potential applications are described.

Tags

Micropatterning Assembly 3D Microvessels Engineered Micro-vessels Vascular Physiology Vascular Biology Anathema Cells Tissue Function Vascular Diseases Organ Systems Disease States Cell Types Matrix Composition Media Composition Vessel Quality Fabrication Injection Molding Assembly Steps PDMS Molds PMMA Housing Pieces Autoclaving Sterilization
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