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Bioengineering

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JoVE Journal Bioengineering
Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair
 

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

Article DOI: 10.3791/54487-v • 07:13 min • October 7th, 2016
October 7th, 2016 •
1, 2, 2, 2,3
1Department of Pathology, University of Virginia, 2Department of Biomedical Engineering, University of Virginia, 3Department of Orthopaedic Surgery, University of Virginia

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We describe an in vivo protocol to measure dorsiflexion of the foot following stimulation of the peroneal nerve and contraction of the anterior crural compartment of the rat hindlimb. Such measurements are an indispensable translational tool for evaluating skeletal muscle pathology and tissue engineering approaches to muscle repair and regeneration.

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Keywords: In Vivo Functional Testing Rat Tibialis Anterior Tissue Engineered Skeletal Muscle Repair Muscle Function Testing Muscle Damage And Recovery Muscle Regeneration Muscle Mass And Force Anesthesia Rat Positioning Electrode Placement Foot Pedal Leg Stabilization
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