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Immunology and Infection

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JoVE Journal Immunology and Infection
Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
 

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Article DOI: 10.3791/55212-v • 08:13 min • December 27th, 2016
December 27th, 2016 •
1, 1, 2, 1
1Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine, Wright State University, 2Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University

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T-lymphocyte mitogenesis is accompanied by blastogenic transformation, whereupon the cell volume enlarges before cell division. Here, we describe a method to quantify blastogenesis in T lymphocytes using an automated cell counter with the capability of measuring cell diameters.

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Rapid Quantification Mitogen-induced Blastogenesis T Lymphocytes Immunomodulatory Drugs Automated Cell Counter Blast Transformation T Lymphocyte Activation Cell Diameters T Lymphocyte Proliferation Assay Immunomodulatory Drug Treatment Cellular Blastogenesis Measurement Mouse Sacrifice Peritoneum Incision Chlorhexidine Application Spleen Removal DPBS Container Complete RPMI 1640 Medium
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