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Immunology and Infection

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JoVE Journal Immunology and Infection
Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
 

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Article DOI: 10.3791/64949-v • 06:22 min • February 10th, 2023
February 10th, 2023 •
*1, *2,3, *2,3, 1, 1, 2,3, 2,3
1Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd, 2Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 3Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences
* These authors contributed equally

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résumé
Automatic Translation

In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers.

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Keywords: Flow Cytometry Standardization Transfer Lymphocytes Japanese Encephalitis Vaccination Cytometer Configuration Compensation Performance Check Compensation Controls CST Beads
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