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JoVE Journal Genetics
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
 

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Article DOI: 10.3791/55528-v • 12:33 min • July 28th, 2017
July 28th, 2017 •
*1,2, *1,3, 1,3
1Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science, University of Minnesota, 3Department of Plant Pathology, University of Minnesota
* These authors contributed equally

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résumé
Automatic Translation

The comparison and optimization of two plant organellar DNA enrichment methods are presented: traditional differential centrifugation and fractionation of the total gDNA based on methylation status. We assess the resulting DNA quantity and quality, demonstrate performance in short-read next-generation sequencing, and discuss the potential for use in long-read single-molecule sequencing.

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Keywords: Plant Organelle DNA Enrichment Next-generation Sequencing Differential Centrifugation Meta Fractionation Wheat Seedling Organelle Isolation STE Buffer Tissue Grinding Filtration
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