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Genetics

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JoVE Journal Genetics
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
 

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Article DOI: 10.3791/59771-v • 11:04 min • May 19th, 2019
May 19th, 2019 •
1, 1, 1, 1,2, 1,3, 1,4
1Tulane Cancer Center, Tulane University, 2Department of Pathology, Tulane University, 3Department of Structural and Cellular Biology, Tulane University, 4Department of Epidemiology, Tulane University

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Here, we present a bioinformatic approach and analyses to identify LINE-1 expression at the locus specific level.

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Keywords: RNA-sequencing Next-generation Sequencing Bioinformatics Pipeline LINE-1 Mobile Elements Genetic Instability L1 Transcripts Cytoplasmic RNA Polyadenylated Transcripts Alignment Strategy Strand Separation Read Counts L1 Loci
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