Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Preparation and In Vitro Characterization of…
JoVE Journal
Medicine

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Medicine
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
 

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Article DOI: 10.3791/55567-v • 09:58 min • May 2nd, 2017
May 2nd, 2017 •
1, 1, 2, 3, 4, 1, 1
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, 2Physikalisch-Technische Bundesanstalt, 3Department of Radiology and Neuroradiology, Ernst-Moritz-Arndt-University Greifswald, 4Electron Microscopy Center, University of Rostock

Chapitres

résumé
Automatic Translation

This manuscript describes the efficient, non-viral delivery of miR to endothelial cells by a PEI/MNP vector and their magnetization. Thus, in addition to genetic modification, this approach allows for magnetic cell guidance and MRI detectability. The technique can be used to improve the characteristics of therapeutic cell products.

Tags

Keywords: Magnetized Cells MiR-modified Cells Endothelial Cells Cell Magnetization MicroRNA Transfection Polyethyleneimene Super Paramagnetic Iron Oxide Nanoparticles Cell Therapy Regenerative Medicine Cell Retention Cell Survival Confocal Microscopy Structured Illumination Microscopy
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter