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Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
 

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Article DOI: 10.3791/55617-v • 11:06 min • May 2nd, 2017
May 2nd, 2017 •
1, 2, 2, 2, 1, 2, 2, 2, 2, 1, 2
1Department of Pharmaceutics, Washington National Primate Research Center, University of Washington, 2Division of Primate Resources, Washington National Primate Research Center, University of Washington

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Here, we describe a minimally invasive laparoscopic technique for serial sampling of liver and mesenteric lymph nodes (MLN) in macaques that allows for increased sampling frequency, and reduces the potential for surgical complications when compared to performing a laparotomy.

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Keywords: Laparoscopic Technique Serial Collection Liver Mesenteric Lymph Nodes Macaques Minimally Invasive GI Tract Microbial Translocation HIV Biology Immunological Consequences Pathology Multiple Time Points Animal Welfare Sterile Procedure Scalpel Incision Varus Needle Pneumoperitoneum CO2 Insufflator
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