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Biochemistry

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JoVE Journal Biochemistry
Bestemmelse af høj-affinitet antistof-antigen Bindende Kinetics Brug Fire Biosensor Platforme
 
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Bestemmelse af høj-affinitet antistof-antigen Bindende Kinetics Brug Fire Biosensor Platforme

Article DOI: 10.3791/55659-v • 15:27 min • April 17th, 2017
April 17th, 2017 •
1, 2, 1, 1
1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science, Columbia University

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Vi beskriver her protokoller til måling af antistof-antigen-bindingsaffinitet og kinetik ved anvendelse af fire almindeligt anvendte biosensor platforme.

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Biokemi optiske biosensorer antistof-antigen-interaktioner bindingskinetik lægemiddelforskning overfladeplasmonresonans BioLayer interferometri
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