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JoVE Journal Developmental Biology
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
 

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes

Article DOI: 10.3791/55736-v • 09:59 min • June 3rd, 2017
June 3rd, 2017 •
*1, *1, 1, 1, 1
1Institute of Cellular and System Medicine, National Health Research Institutes
* These authors contributed equally

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résumé
Automatic Translation

This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.

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Keywords: Extracellular Vesicles Exosomes Mammary Basal Epithelial Cells Mammary Luminal Cells Stem Cell Properties Ultracentrifugation Nanoparticle Tracking Analysis Iodixanol Density Gradient
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