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Developmental Biology

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JoVE Journal Developmental Biology
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
 

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Article DOI: 10.3791/61086-v • 08:37 min • October 9th, 2020
October 9th, 2020 •
1, 1
1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center, Baylor College of Medicine

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We describe a technique for profiling microRNAs in early mouse embryos. This protocol overcomes the challenge of low cell input and small RNA enrichment. This assay can be used to analyze changes in miRNA expression over time in different cell lineages of the early mouse embryo.

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Keywords: Small RNA Sequencing MicroRNAs Embryonic Development Low Input Samples Embryo Dissection Embryo Staging Cell Suspension Cell Sorting RNA Extraction
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