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Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
 

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Article DOI: 10.3791/55870-v • 06:26 min • June 19th, 2017
June 19th, 2017 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), 2Department of Cardiac Surgery, University of Rostock, 3Department of Life, Light and Matter of the Interdisciplinary Faculty, University of Rostock

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Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.

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Keywords: Gap Junction Micro RNA 3D-FRAP Microscopy Cardiomyocytes Cell Culture Electroporation Confocal Microscopy Fluorescence Recovery After Photobleaching (FRAP)
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