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Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
 

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Article DOI: 10.3791/56141-v • 12:38 min • December 10th, 2017
December 10th, 2017 •
*1, *1, 2, 1
1Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University, 2Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University
* These authors contributed equally

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résumé
Automatic Translation

Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.

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Keywords: 3D Reconstruction Vascular Architecture CLARITY-cleared Mouse Ovary Follicle Development Somatic Cells Vascular System Nervous System Passive CLARITY Image Analysis Imaris Perfusion Hydrogel Tissue Fixation Ovarian Bursa
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