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JoVE Journal Cancer Research
Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
 

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Article DOI: 10.3791/56332-v • 07:44 min • March 12th, 2018
March 12th, 2018 •
1,2, 2,3
1Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2BC Cancer Agency, Provincial Health Services Authority, 3Department of Pathology and Laboratory Medicine, University of British Columbia

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The goal of this article is to provide a detailed description of the protocol for the pulmonary metastasis assay (PuMA). This model permits researchers to study metastatic osteosarcoma (OS) cell growth in lung tissue using a widefield fluorescence or confocal laser-scanning microscope.

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Metastatic Lung Colonization Osteosarcoma Pulmonary Metastasis Assay Visualize Study Growth Metastatic Cells Lung Microenvironment Advantage Technique Three-dimensional Microenvironment Cannulating Trachea Insufflating Lung Tail Vein Injection Tumor Cells Mouse Euthanasia Dorsal Recumbency Dissect Sternum Thoracic Inlet Puncturing Lungs Muscle And Connective Tissue Successful Cannulation 20 Gauge IV Catheter Catgut Suture
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