Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Semi-quantitative Detection of RNA-dependent RNA…
JoVE Journal
Genetics

Ce contenu est en libre accès.

JoVE Journal Genetics
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
 

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Article DOI: 10.3791/57021-v • 08:26 min • June 12th, 2018
June 12th, 2018 •
*1, *1, 1, 1, 1
1Division of Cancer Stem Cell, National Cancer Center Research Institute
* These authors contributed equally

Chapitres

résumé
Automatic Translation

Human telomerase reverse transcriptase (TERT) synthesizes not only telomeric DNA but also double-stranded RNA through RNA-dependent RNA polymerase activity. Here, we describe a newly established assay to detect RNA-dependent RNA polymerase activity of endogenous TERT.

Tags

RNA-dependent RNA Polymerase Activity Human Telomerase Reverse Transcriptase Protein RNA Biology Biological Significance RdRP Activity HeLa Cells Lysis Buffer A Sonication Protein A-agarose Beads
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter