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Biochemistry

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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
 

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Article DOI: 10.3791/57051-v 06:18 min March 13th, 2018
March 13th, 2018

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résumé

Current multiplexed diagnostics to detect Zika, chikungunya, and dengue viruses require complex sample preparation and expensive instrumentation, and are difficult to use in low resource environments. We show a diagnostic that uses isothermal amplification with target-specific strand displaceable probes to detect and differentiate these viruses with high sensitivity and specificity.

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Keywords: Multiplexed Isothermal Amplification Point-of-care Diagnostics Zika Chikungunya Dengue Loop-mediated Isothermal Amplification RT-LAMP Aedes Aegypti Mosquitoes Urine Sample 3D Printed Observing Box
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