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Biochemistry

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JoVE Journal Biochemistry
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
 

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Article DOI: 10.3791/59925-v • 07:15 min • January 2nd, 2020
January 2nd, 2020 •
1, 2, 2, 3
1PICT Platform, Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille

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This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections.

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Keywords: Fluorescent Probes Lignin Plant Sections SFLIM Native Autofluorescence FRET Analysis Lignocellulosic Biomass Enzyme Activity Polysaccharides Sample Sectioning Multidimensional Microscopy PEG Embedding Microtome Fluorescent Staining Spectral Window SFLIM Detector Titanium-doped Sapphire Laser
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