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Genetics

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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) Histone endringer fra Saccharomyces cerevisiae
 
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Chromatin Immunoprecipitation (ChIP) Histone endringer fra Saccharomyces cerevisiae

Article DOI: 10.3791/57080-v • 11:07 min • December 29th, 2017
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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Her beskriver vi en protokoll for chromatin immunoprecipitation av modifisert histones fra spirende gjær Saccharomyces cerevisiae. Immunoprecipitated DNA blir brukt i kvantitative PCR avhøre overflod og lokalisering av histone post-translasjonell endringer i genomet.

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Genetikk problemet 130 Epigenetics chromatin histone modifikasjoner metylering acetylation chromatin immunoprecipitation gjær
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