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Medicine

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JoVE Journal Medicine
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
 

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Article DOI: 10.3791/57319-v • 07:32 min • March 6th, 2018
March 6th, 2018 •
*1,2,3, *1,2, 2,4, 2,4, 1,2, 1,2
1Department of Internal Medicine, MUMC, 2CARIM, MUMC, 3Department of Immunology and Biochemistry, Biomedical Research Institute, Hasselt University, 4Department of Pathology, MUMC
* These authors contributed equally

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résumé
Automatic Translation

This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.

Tags

Keywords: Adipose Tissue Flow Cytometry Stromal Vascular Fraction Immune Cells Phenotyping Metabolic Research Obesity Inflammation Cell Isolation Cell Staining
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