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Genetics

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JoVE Journal Genetics
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
 

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Article DOI: 10.3791/57386-v • 09:00 min • May 2nd, 2018
May 2nd, 2018 •
1,2, 3, 4
1Department of Animal Health, Universidad Complutense de Madrid, 2Visavet Health Surveillance Centre, Universidad Complutense de Madrid, 3Department of Zoology, University of Oxford, 4Department of Microbiology, Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

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résumé
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Here we present an experimental method to test the role of multicopy plasmids in the evolution of antibiotic resistance.

Tags

Multicopy Plasmids Evolution Antibiotic Resistance Experimental Method Microbiology Bacterial Innovations Molecular Biology Methods Simple Experimental Design E Coli MG1655 Strain Antibody Resistance Gene Lambda Phage ATTB Site PCR Amplification Isothermal Assembly Electrocompetent Cells Electroporation LB Broth Arabinose Microfuge Tube Culture LB Agar Plate
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