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Immunology and Infection

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JoVE Journal Immunology and Infection
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
 

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Article DOI: 10.3791/57780-v • 12:36 min • June 26th, 2018
June 26th, 2018 •
*1, *1, 1, 2, 1,3
1Department of Immunology and Microbiology, University of Colorado School of Medicine, 2OMNI Biomarkers, Development Sciences, Genentech, 3Department of Pediatrics, Division of Allergy and Immunology, University of Colorado School of Medicine
* These authors contributed equally

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Here we describe a single-cell proteomic approach to evaluate immune phenotypic and functional (intracellular cytokine induction) alterations in peripheral whole blood samples, analyzed via mass cytometry.

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Single-cell Analysis Immunophenotype Cytokine Production Mass Cytometry Autoimmunity Cell Frequency Abnormalities Function Differences Autoimmune Disease Peripheral Blood Sample Blood Processing Steps Antibody Panel Design Barcoding Process High Dimensional Single Cell Data Systems Immunology Approach SLE (systemic Lupus Erythematosus) Immune Dysregulation Round Bottom Polystyrene Tubes RPMI (Roswell Park Memorial Institute) Ruxolitinib
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