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Immunology and Infection

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JoVE Journal Immunology and Infection
全血流式细胞术分析人单核细胞亚群的表征
 
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全血流式细胞术分析人单核细胞亚群的表征

Article DOI: 10.3791/57941-v • 09:12 min • October 17th, 2018
October 17th, 2018 •
1,2, 1,2, 3, 4, *1,2, *1,2
1Department of Surgery, Vascular Biology Research Centre, Westmead Hospital, 2Westmead Clinical School, Department of Surgery, The University of Sydney, 3Westmead Research Hub, Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research, Westmead Hospital
* These authors contributed equally

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在这里, 我们提出了一个通过全血流式细胞仪表征单核细胞子集的协议。这包括概述如何门的子集和评估他们的表面标记的表达, 并提供了一个例子, 评估 M1 (炎症) 和 M2 标记 (抗炎) 的表达。

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免疫学与感染 140 期 单核细胞 免疫学 流式细胞术 炎症 单核细胞门控 单核细胞标记表达
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