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Developmental Biology

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JoVE Journal Developmental Biology
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
 

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Article DOI: 10.3791/58034-v • 08:48 min • August 16th, 2018
August 16th, 2018 •
1, 1, 1
1Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute

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In this protocol, we aim to describe a reproducible method for combining dissociated human pluripotent stem cell derived neurons and astrocytes together into 3D sphere cocultures, maintaining these spheres in free floating conditions, and subsequently measuring synaptic circuit activity of the spheres with immunoanalysis and multielectrode array recordings.

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Keywords: Synaptic Microcircuit 3D Cocultures Astrocytes Neurons Human Pluripotent Stem Cells Neural Cell Types Synaptic Circuit Formation Neuroscience Organoid Technologies Drug Development Neuroregeneration Neural Progenitors Astrocyte Progenitors Astrocyte Subtypes Neural Induction Neural Medium Rho-kinase Inhibitor ECM-coated Plates Cell Aggregation Cell Dissociation Cell Identity Confirmation Cryo-preservation
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