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Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
 

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Article DOI: 10.3791/58066-v • 11:02 min • September 14th, 2018
September 14th, 2018 •
1
1Medical Research Council Laboratory of Molecular Biology

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Incorporating a cyclopropene derivative of lysine into antibodies allows the site-specific, rapid and efficient linkage of tetrazine-bearing molecules to generate antibody-drug conjugates.

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Genetic Encoding Non-canonical Amino Acid Antibody-drug Conjugates Bioorthogonal Reaction Protein Modification Antibody Production Psychodision Reaction Linkage Stability HEK Suspension Cells Cyclopropene Derivative Of Lysine Sodium Hydroxide HCl DNA Transfection Reagent Mixture Trastuzumab Supernatant Bead Wash Buffer Protein A Resin
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