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Bioengineering

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JoVE Journal Bioengineering
Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
 

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Article DOI: 10.3791/58293-v • 07:41 min • October 17th, 2018
October 17th, 2018 •
1,2, 1,2,3, 1,2, 1,2,3, 1,2, 4, 5, 1,2, 1,2, 1,2
1The Jake Gittlen Laboratories for Cancer Research, Penn State College of Medicine, 2Division of Experimental Pathology, Department of Pathology, Penn State College of Medicine, 3Medical Scientist Training Program, Penn State College of Medicine, 4Center for In Vivo Microscopy, Duke University Medical Center, 5KTM Research

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We developed protocols and designed a custom apparatus to enable embedding of millimeter-scale specimens. We present sample preparation procedures with an emphasis on embedding in acrylic resin and polyimide tubing to achieve rigid immobilization and long-term storage of specimens for the interrogation of tissue architecture and cell morphology by micro-CT.

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Rigid Embedding Fixed Specimens Stained Specimens Whole Millimeter-scale Specimens Section-free 3D Histology Micro-computed Tomography Three-dimensional Histological Images Stable Samples Long-term Preservation Multiple Imaging Methods High Throughput Model System Phenomics Toxicology Human Tissue Diagnostics Sample Orientation Sample Maneuvering Visual Demonstration Zebrafish Starve Period Fixation Process NBF Solution Glass Vials Overnight Fixation Sample Rinsing
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