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Immunology and Infection

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JoVE Journal Immunology and Infection
"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
 

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Article DOI: 10.3791/58333-v • 10:25 min • February 19th, 2019
February 19th, 2019 •
1, 1, 1, 1, 1, 1
1Section of Virology, Department of Medicine, Imperial College

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The goal of this protocol is to provide a step-by-step guide to perform 3-D "liver-on-a-chip" infection experiments with the hepatitis B virus.

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Keywords: Liver-on-a-chip Primary Hepatocytes Kupffer Cells Hepatitis B Virus Viral Pathogenesis Infection Kinetics Viral Replication Immune Invasion Physiological Model Drug Testing Hepatic Microenvironment Hepatotropic Infections Liver Diseases Drug Metabolism Long-term Culture Specialized Tools Visual Demonstration Membrane Microfluidic Plates Seeding Medium Priming
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