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Immunology and Infection

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JoVE Journal Immunology and Infection
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
 

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Article DOI: 10.3791/58457-v • 06:16 min • October 18th, 2018
October 18th, 2018 •
1, 1
1Department of Oral Biology, University at Buffalo

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We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images.

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Keywords: Real-time Imaging Fungal Biofilm Two-phase Recirculating Flow System Fluid Flow Biofilm Growth And Development Flow Apparatus Bubble Trap Temperature Probe Filter Growth Flask YPD Media Pump Slide Adapter Microscope
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