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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
 

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Article DOI: 10.3791/51766-v • 11:26 min • August 14th, 2014
August 14th, 2014 •
*1, *1, 1, 1
1Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam
* These authors contributed equally

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résumé
Automatic Translation

Leukocytes cross the endothelial monolayer using the paracellular or the transcellular route. We developed a simple assay to follow the distribution of endogenous junctional VE-cadherin and PECAM-1 during leukocyte transendothelial migration under physiological flow to discriminate between the two transmigration routes.

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Real-time Imaging Endothelial Cell-cell Junctions Neutrophil Transmigration Physiological Flow Inflammation Leukocyte Transendothelial Migration Paracellular Route Transcellular Route In Vitro Assay VE-cadherin PECAM-1 Fluorescently-labeled Antibodies Electrical Cell-substrate Impedance Sensing FRAP Measurements Transendothelial Migration Flow Conditions Leukocytes Endothelium
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