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Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
 

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

Article DOI: 10.3791/58470-v • 08:40 min • February 14th, 2019
February 14th, 2019 •
1, 2
1Department of Plant Biology, Michigan State University, 2Department of Chemistry, Michigan State University

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Here we present a protocol to separate solubilized thylakoid complexes by Native Green Gel electrophoresis. Green gel bands are subsequently characterized by Time Correlated Single Photon Counting (TCSPC) and basic steps for data analysis are provided.

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Keywords: Thylakoid Protein Complexes Native Green Gel Electrophoresis Time-correlated Single Photon Counting Photosynthesis Research Chlorophyll Spinach Leaves TMK Buffer Methanol Extraction Centrifugation Sample Preparation
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