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Genetics

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JoVE Journal Genetics
En passelig protokoll for streng montering gRNA kloning (STAgR)
 
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En passelig protokoll for streng montering gRNA kloning (STAgR)

Article DOI: 10.3791/58556-v • 10:00 min • December 26th, 2018
December 26th, 2018 •
1,2, 1,2, 3, 3, 2,4, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

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Her presenterer vi streng montering gRNA kloning (STAgR), en metode for å enkelt multiplex gRNA vektorer for CRISPR/Cas9 nærmer seg. STAgR gjør gRNA multipleksing enkel, effektiv og kan tilpasses.

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Genetikk problemet 142 CRISPR Cas9 dCas9 gRNA kloning gRNA multipleksing genomet redigering redigering av transcriptome
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