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JoVE Journal Genetics
Direkte Gene knock-out av Axolotl spinal Cord nevrale stamceller via electroporation av CAS9 protein-gRNA komplekser
 
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Direkte Gene knock-out av Axolotl spinal Cord nevrale stamceller via electroporation av CAS9 protein-gRNA komplekser

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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Presentert her er en protokoll for å utføre tid-og plassbegrensede genet Knock-Out i Axolotl spinal snorer ved å injisere CAS9-gRNA kompleks i ryggmargen sentrale kanalen etterfulgt av electroporation.

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Genetikk Axolotl ryggmargen regenerering CRISPR-Cas9 nevrale stamceller gen Knock-Out
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