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Genetics

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JoVE Journal Genetics
CAS9 protein-gRNA kompleksleri Electroporation yoluyla Axolotl omurilik nöral kök hücrelerinin doğrudan gen Knock-Out
 
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CAS9 protein-gRNA kompleksleri Electroporation yoluyla Axolotl omurilik nöral kök hücrelerinin doğrudan gen Knock-Out

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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Burada sunulan, CAS9-gRNA kompleksini omurilik merkezi kanalına enjekte ederek, akolotl spinal tellerinde zaman ve uzaya kısıtlı gen vuruş yapmak için bir protokoldür ve ardından Electroporation.

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Genetik sayı 149 Axolotl omurilik rejenerasyon CRISPR-Cas9 nöral kök hücreler gen Knock-Out
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