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Biochemistry

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JoVE Journal Biochemistry
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
 

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Article DOI: 10.3791/58617-v • 09:06 min • October 24th, 2018
October 24th, 2018 •
1, 1, 1, 1, 1, 1,3, 1,2, 1
1Departments of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, 2Department of Medicine, Johns Hopkins School of Medicine, 3Department of Mental Health, Johns Hopkins School of Public Health

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Here, we describe the protocol and implementation of Methyl-Seq, an epigenomic platform, using a rat model to identify epigenetic changes associated with chronic stress exposure. Results demonstrate that the rat Methyl-Seq platform is capable of detecting methylation differences that arise from stress exposure in rats.

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Keywords: Rat Methyl-Seq DNA Methylation Epigenomics Environmental Factors Physiological Processes Stress Exposure Sequencing-based Tool Array-based Platforms DNA Shearing Magnetic Beads DNA-binding Adapter Ligation Methyl-Seq Block Mix Capture Library Hybridization Mix Streptavidin Magnetic Beads
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