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Developmental Biology

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JoVE Journal Developmental Biology
Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
 

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Article DOI: 10.3791/58678-v • 06:47 min • November 26th, 2018
November 26th, 2018 •
1, 1, 1,2, 1
1Department of Anatomy and Embryology, Leiden University Medical Center, 2Department of Applied Stem Cell Technologies, University of Twente

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This step-by-step protocol provides a detailed description of the experimental setup and data analysis for the assessment of inflammatory responses in hiPSC-ECs and the analysis of leukocyte adhesion under physiological flow.

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Microfluidic Assay Leukocyte Adhesion Human Induced Pluripotent Stem Cells Endothelial Cells HiPSC-ECs Experimental Setup Data Analysis Microfluidic Chip Coating Fibronectin Working Solution Cell Suspension Microscope Examination
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