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Neuroscience

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JoVE Journal Neuroscience
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
 

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Article DOI: 10.3791/58974-v • 10:57 min • June 6th, 2019
June 6th, 2019 •
1, 1, 2, 3, 1
1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

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Automatic Translation

This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.

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Primary Cell Culture Purified Neurons GABAergic Neurons Glutamatergic Neurons Fluorescence-activated Cell Sorting Electrophysiological Analysis Morphological Analysis Survival Analysis Neuronal Physiology Synapse Formation Neuronal Networks Cortical Glutamatergic Principal Cells GABAergic Interneurons Fluorescent Proteins Glass Coverslips PLL Solution Filter Sterilize Solution
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