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Immunology and Infection

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JoVE Journal Immunology and Infection
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
 

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Article DOI: 10.3791/59357-v • 07:20 min • April 9th, 2019
April 9th, 2019 •
*1, *2, 1, 1
1Department of Internal Medicine, University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology, Johns Hopkins University School of Medicine
* These authors contributed equally

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résumé
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Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

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Keywords: Colonoid Monolayers Intestinal Epithelium Host-pathogen Interaction Mucus Biology Microbiome Inflammatory Bowel Disease 3D Organoid Culture Ex Vivo Studies Harvesting Solution Cell Suspension DMEM/F12 Medium Cell Plating Expansion Medium
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