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Immunology and Infection

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JoVE Journal Immunology and Infection
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
 

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Article DOI: 10.3791/59531-v • 10:13 min • May 6th, 2019
May 6th, 2019 •
1, 1, 1,2,3,4
1Department of Microbiology and Immunology, Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy, Western University, 3Department of Surgery, Division of General Surgery, Western University, 4Lawson Health Research Institute

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We describe here a flow cytometry-based in vivo killing assay that enables examination of immunodominance in cytotoxic T lymphocyte (CTL) responses to a model tumor antigen. We provide examples of how this elegant assay may be employed for mechanistic studies and for drug efficacy testing.

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In Vivo Cytotoxicity Assays Immunodominance Tumor-specific CD8+ T Cell Responses Peptide Epitopes Tumor Antigens Secondary Lymphoid Organ Killing Assay Cytotoxic T Cell Responses Vaccine Design Immunotherapeutic Protocols Aseptic Technique Mouse Tissue Handling Target Cell Preparation Tail Vein Injections T-antigen-positive Adherent Tumor Cell Line Trypsin EDTA
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