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Neuroscience

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JoVE Journal Neuroscience
Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
 

Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

Article DOI: 10.3791/59695-v • 09:25 min • May 21st, 2019
May 21st, 2019 •
1, 2, 1,2
1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 2School of Neuroscience, Virginia Polytechnic Institute and State University

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This protocol is designed to efficiently quantify ubiquitin-proteasome system (UPS) activity in different cellular compartments of the rodent brain. Users are able to examine UPS functioning in nuclear, cytoplasmic and synaptic fractions in the same animal, reducing the amount of time and number of animals needed to perform these complex analyses.

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Subcellular Fractionation Ubiquitin-proteasome System Protein Ubiquitination Proteasome Activity Rodent Brain Cytoplasmic Fraction Nuclear Fraction Synaptic Fraction Brain Tissue Homogenization Centrifugation
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