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Immunology and Infection

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JoVE Journal Immunology and Infection
Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
 

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

Article DOI: 10.3791/59731-v • 06:47 min • May 24th, 2019
May 24th, 2019 •
1, 1
1Division of Immunology, Allergy, and Rheumatology, Department of Internal Medicine, College of Medicine, University of Cincinnati

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résumé
Automatic Translation

Here, we present a protocol to prepare apoptotic thymocytes and peritoneal macrophages and analyze the efficiency of efferocytosis and the specific inhibitor-mediated blocking of apoptotic thymocytes engulfment. This protocol has a broad application in cell-mediated clearance of other particles including artificial beads and bacteria.

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Keywords: Apoptotic Cells Thymocyte Engulfment Macrophages Phagocytic Cell Types Apoptotic Cell Binding Cell Debris Cell Clearance CFSE Labeling Staurosporine Peritoneal Macrophages Thioglycollate Cell Isolation Tissue Culture
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