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Immunology and Infection

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JoVE Journal Immunology and Infection
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
 

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Article DOI: 10.3791/53424-v • 07:45 min • November 28th, 2015
November 28th, 2015 •
1, 1, 1
1Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam

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Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.

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Metabolic Characterization Polarized M1 Macrophages Polarized M2 Macrophages Bone Marrow-derived Macrophages Real-time Extracellular Flux Analysis Metabolic Pathways Macrophage Subsets Pro-inflammatory Alternative Macrophage Activation Resolution Of Inflammation Wound Healing Metabolic Reprogramming Extracellular Flux Analyzer PH Sensors Oxygen Sensors Extracellular Acidification Rate (ECAR) Oxygen Consumption Rate (OCR) Glycolytic Metabolism Mitochondrial Activity Mitochondrial Oxidative Phosphorylation (OXPHOS) Spare Respiratory Capacity (SRC) Cytokines Pharmacologic Interventions
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