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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
 

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Article DOI: 10.3791/59868-v • 08:03 min • July 15th, 2019
July 15th, 2019 •
1, 1
1Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine

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We present a method for isolating and cultivating primary human salivary gland-derived epithelial cells. These cells exhibit gene expression patterns consistent with them being of salivary epithelial origin and can be grown as salispheres on basement membrane matrices derived from Engelbreth-Holm-Swarm tumor cells or as monolayers on treated culture dishes.

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Isolation Salivary Epithelial Cells Human Salivary Glands In Vitro Growth Salispheres Monolayers Primary Cellular Models Organoid-like Structures Biology Human Viruses Replication Salivary Gland Horizontal Transmission Salisphere-based System Cellular Models In Vivo Type Situation Mouse Primary Submandibular Gland Autoclaved Sterilized Dissecting Scissors Surgical Forceps Mince Salivary Gland Tissue Dispase/Collagenase Solution Incubate At 37 Degrees Celsius
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