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Developmental Biology

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JoVE Journal Developmental Biology
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
 

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Article DOI: 10.3791/60112-v • 11:42 min • November 4th, 2019
November 4th, 2019 •
1,2,3, 3, 4, 5,6, 1,2,3
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 2Wallenberg Center for Molecular, Lund University, 3Center for Neuroscience and Cell Biology, University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

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This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.

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Keywords: Hemogenic Reprogramming Human Fibroblasts Transcription Factors Lentiviral Production Fibroblast Transduction Cell Culture HEK293T Cells Transfer Plasmids Packaging Vector Envelope Vector Calcium Chloride Hematopoietic Stem And Progenitor Cells Stem Cell Transplantation
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