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Developmental Biology

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JoVE Journal Developmental Biology
Hemogena omprogrammering av humana fibroblaster genom påtvingat uttryck av transkriptionsfaktorer
 
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Hemogena omprogrammering av humana fibroblaster genom påtvingat uttryck av transkriptionsfaktorer

Article DOI: 10.3791/60112-v • 11:42 min • November 4th, 2019
November 4th, 2019 •
1,2,3, 3, 4, 5,6, 1,2,3
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 2Wallenberg Center for Molecular, Lund University, 3Center for Neuroscience and Cell Biology, University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

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Detta protokoll visar induktion av en hemogena program i human dermal fibroblaster genom påtvingat uttryck av transkriptionsfaktorerna GATA2, GFI1B och FOS för att generera hematopoietiska Stem och progenitorceller.

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Utvecklingsbiologi hemogena omprogrammering fibroblast transkriptionsfaktorer hematopoies hematopoietiska Stem och stamceller endotelial-till-hematopoietisk övergång
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